FastQCFastQC Report
Wed 24 May 2017
G153-M5-Index-2_CGATGT-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M5-Index-2_CGATGT-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16553629
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN375770.22700158376148216No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT174360.10533037800955912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC14550.016.6325863
CCACTAG38650.014.5151263
CACGATC19500.014.4390331
ACGATCA18750.013.2580872
CACTAGC53400.010.75488954
TAGCCTC80350.010.4861632
TTAGATC23300.010.3858122
TCTTCGT81150.010.05715644
CCCACTA58300.09.8486252
TAGATCC22300.09.7669833
CGCAATT18550.09.7237261
CTCTTCG85100.09.66801443
CTAGCCT92450.09.6601061
TAACGCG2302.5702367E-49.5670684
CGACAAT10650.09.5010371
TAGCTCG33700.09.3994122
GCATATC26100.09.35574156
ACGCAAC9100.09.185657510
AATCGAA12950.09.0047815
ATAGTTA27850.09.00028615
Produced by FastQC (version 0.11.2)