FastQCFastQC Report
Wed 24 May 2017
G153-M5-Index-2_CGATGT-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M5-Index-2_CGATGT-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16553629
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT29000.017.222431
CGAATGT23900.016.6584433
TCGGTTT31900.015.3079162
ACCGGTA19100.015.20333727
GTCGGTT33250.014.82167151
ACGTGCC26000.014.8048520
AGTTCCG44800.014.63310826
TCCGCCC45650.014.55340229
TTCCGCC47150.014.183722528
GTTCCGC46800.014.14877927
AACGTGC27650.014.0804819
CCGCCCT47700.013.9740630
AATTGCG21800.013.52156536
CGCCCTG50250.013.17812731
ATTGCGA22550.013.07184537
GCGCATT50400.013.00905644
CGGTTTG34100.012.7721653
CGGGGAA27000.012.3593931
CTCGGTT20350.012.1629121
CGGGCTA9350.012.05431651
Produced by FastQC (version 0.11.2)