FastQCFastQC Report
Wed 24 May 2017
G153-M4-Index-1_ATCACG-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M4-Index-1_ATCACG-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14867384
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT27800.014.3054341
CGAATGT24050.014.0851923
TAAGGCG45000.013.589479
TACGTCA25250.013.1566632
CGGGCTA8950.013.0835671
AATCCGA22050.013.06968324
GTCGGAT9600.012.657991
ACGTGCC22550.012.58295720
ATCCGAA22400.012.57092525
TCCGCCC36750.012.51106529
AGTTCCG36250.012.50148526
GTCGGTT27050.012.4967611
ACCGGTA16100.012.43428427
GTTCCGC36600.012.38201827
ATACGTC26700.012.27719931
TCGGTTT26550.012.2617192
CCGCCCT37700.012.02073930
GTCGATT11600.011.9992991
GCGCATT37050.011.99557444
AACGTGC23400.011.93788619
Produced by FastQC (version 0.11.2)