FastQCFastQC Report
Wed 24 May 2017
G153-M16-Index-8_ACTTGA-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M16-Index-8_ACTTGA-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17045958
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT414000.24287282650819628No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC397330.2330933820205353No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC352580.20684082408275323No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT342520.20093913172847194No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268460.1574918816531168No Hit
CAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG230720.1353517355844711No Hit
AAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCAT215510.12642879913232216No Hit
GAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCA200320.11751759566696104No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC171220.1004460998906603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA18250.022.6391121
TCGGTTT33800.016.3996732
GTCGGTT33650.015.4299111
AATCCGA22150.014.30189624
ACCGGTA21100.014.17942927
ATCCGAA22150.014.10342425
CGGTTTG36000.013.99222853
CGAATGT25050.013.961863
TACGTCA130700.013.80280532
ATACGTC131750.013.65940431
CTCGAAT30000.013.5511831
ACGTCAC134400.013.42281633
ATTGGAC152600.013.3763756
CATACGT136850.013.32637530
TCATACG136700.013.30881329
AAATCCG24400.013.16316223
CGTCACA139150.013.01040834
AATTGGA171600.012.71548755
GAATGCA162500.012.64510932
CCGGGAT21550.012.4056271
Produced by FastQC (version 0.11.2)