FastQCFastQC Report
Wed 24 May 2017
G153-M15-Index-7_CAGATC-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M15-Index-7_CAGATC-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18779841
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN423470.2254917919699107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC37050.024.1049371
ACGATCA39600.021.8879592
CGATCAC42500.020.344413
TAATGTA66700.011.5467384
TCACAAG81500.011.2823216
CCACTAG41300.011.1869253
AATGTAG69850.011.0247495
TTAGATC26400.010.9162032
TAGCCTC80650.010.7744962
TCTTCGT76600.010.65486644
CCCCGTA12800.010.1396247
CCGTTAA13050.010.10885218
CTAGCCT91050.09.977881
ATACGCG2659.712483E-69.96058722
TTATCCG9750.09.9272256
CTTTAAT99300.09.92423251
ACGATCG3553.4309778E-89.9159213
CGGAGAG29800.09.8175591
GGGTAAC83650.09.75588823
ATTAACG53100.09.68915720
Produced by FastQC (version 0.11.2)