FastQCFastQC Report
Wed 24 May 2017
G153-M15-Index-7_CAGATC-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M15-Index-7_CAGATC-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18779841
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT209160.11137474486605078No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC200190.10659834659942008No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC198140.10550675056301062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA13400.015.6652251
ACCGGTA21300.014.76912927
CTCGAAT33650.014.577591
CGAATGT27600.014.345843
TCGGTTT39800.014.3145652
GTCGGTT39100.014.2975481
TACGTCA67750.014.06104332
ATACGTC68550.013.92870631
TCATACG71200.013.59474829
AGTTCCG43450.013.56882626
TCCGCCC43800.013.56088729
AATCCGA25800.013.55749124
CATACGT73000.013.28966930
CGGGGAA31150.013.1938861
GTTCCGC44650.013.15492227
GTCGGAT12450.013.13349151
ATCCGAA26500.013.11638825
ACGTCAC74550.013.01453633
GCGCATT44750.012.979671544
CCGCCCT46150.012.96569130
Produced by FastQC (version 0.11.2)