FastQCFastQC Report
Wed 24 May 2017
G153-M14-Index-27_ATTCCT-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M14-Index-27_ATTCCT-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15258773
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153300.10046679375858072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA9450.016.1329331
ACCGGTA16750.014.31591427
CGAATGT24000.014.1144573
TCGGTTT28600.013.5362992
TACGTCA43850.013.49637532
AATCCGA19900.013.155272524
ATTGCGA17800.013.0994637
ATCCGAA19800.012.99954225
AATTGCG17900.012.90338936
ATACGTC45950.012.83151731
CTCGAAT26000.012.6621941
CGCGTGT4650.012.3542521
GTCGGTT31000.012.3304941
CACCGGT20200.012.30637626
TCATACG49150.012.21930529
AAATCCG21650.012.19308723
TGCGATA19100.012.09215139
AACGTGC19750.012.02810419
CATACGT49800.011.97146630
TTGCGAT19300.011.96727538
Produced by FastQC (version 0.11.2)