FastQCFastQC Report
Wed 24 May 2017
G152-M9-Index-5_ACAGTG-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M9-Index-5_ACAGTG-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15845711
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327970.2069771435311423No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT228080.14393800316060287No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT202530.12781376613520215No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC179680.1133934602240316No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC175420.11070503557713504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA11050.015.1861261
TAAGGCG43350.014.9692079
CGAATGT18650.014.7430083
ACCGGTA19600.014.0297527
CTCGAAT22100.013.7874031
ATTGCGA19900.013.70726937
AATTGCG19900.013.70722636
ATACGCG1302.3358912E-413.5366911
TGCGATA21600.013.03524939
TTGCGAT21300.013.01287538
TCGGTTT30050.012.809982
GTCGGTT30800.012.5453561
CGGGCTA8100.012.5391621
AATCCGA20000.012.53895324
ATCCGAA19850.012.52288425
CGGGGAT18700.012.5158391
CGCGTAA1953.414396E-612.4552871
TTTAGGC53900.012.4470382
CGGCGAT6400.012.4199031
AAATCCG20950.012.389317523
Produced by FastQC (version 0.11.2)