FastQCFastQC Report
Wed 24 May 2017
G152-M8-Index-12_CTTGTA-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M8-Index-12_CTTGTA-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16111499
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164360.10201409564684204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT22450.016.6566873
ACCGGTA18700.016.5874427
AATTGCG19800.015.88753536
ATTGCGA20850.014.77110737
CTCGAAT28700.014.61570551
TAAGGCG50300.014.5189189
TGCGATA21950.014.13073939
TTGCGAT22650.013.59724438
CACCGGT23650.013.30127526
AATCCGA20400.013.15582424
ATCCGAA21050.012.95859725
TCCGCCC41200.012.92171129
GTCGGTT30350.012.6572281
CCGGTAG24950.012.52044328
GCGATAA25150.012.50877540
TCGGTTT29900.012.5064422
GACACCG25350.012.49605524
AAATCCG21500.012.4816523
AGGCGTC57900.012.46118711
GTTCCGC42750.012.35028627
Produced by FastQC (version 0.11.2)