FastQCFastQC Report
Wed 24 May 2017
G152-M7-Index-11_GGCTAC-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M7-Index-11_GGCTAC-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15889036
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN286840.18052699987588927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC8200.012.3410433
CCACTAG36350.011.92256453
TTAGATC20650.010.8662512
CTTAGAT25250.010.1919041
TAGATCC21700.010.1379033
TAGCCTC68900.09.7701642
CGCAATT20300.09.7516321
ATCGCTC15050.09.52064442
CGACGAT6950.09.4943951
TAGCTCG30200.09.46978622
CTAGCCT76700.09.2053551
ACGATCG3655.439924E-79.0407883
CGCGGAT9900.08.887011
CGGAGAG25050.08.8683831
CACGATC14550.08.7679041
CACTAGC48800.08.7005234
CCCACTA48900.08.6375782
CGACAAT9950.08.6212921
GTCGATT3851.1761185E-68.5696161
TCATACG29550.08.55994910
Produced by FastQC (version 0.11.2)