FastQCFastQC Report
Wed 24 May 2017
G152-M7-Index-11_GGCTAC-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M7-Index-11_GGCTAC-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15889036
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168770.1062178976748495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT21500.018.0072753
ACCGGTA19550.014.85296827
CTCGAAT29000.014.767871
ATTGCGA19950.014.33535437
AATTGCG20700.013.9221936
TTGCGAT21200.013.80141938
GTCGGTT27850.013.5545481
CGCGATA7750.013.3878341
TGCGATA21700.013.38157239
TAAGGCG49300.012.9840139
TCGGTTT28700.012.8765982
ACGTTCG2054.4365697E-712.8765586
CACCGGT23050.012.78822826
AGTTCCG43600.012.66379226
AATCCGA22200.012.4851224
ATCCGAA22300.012.42917225
TCCGCCC44800.012.27575129
CGGGGAT17200.012.19296551
AAATCCG23500.012.16811623
GCGCATT45150.012.08637944
Produced by FastQC (version 0.11.2)