FastQCFastQC Report
Wed 24 May 2017
G152-M6-Index-4_TGACCA-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M6-Index-4_TGACCA-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16488692
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN304770.1848357650200513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC12950.014.1012813
CACGATC15900.013.558871
ACGATCA15900.011.4849362
TAGCCTC64350.010.4963282
CCACTAG31350.010.17608453
CGCAATT19550.010.0146961
CGAAAAT32500.09.9501251
TCATACG31600.09.53578510
TTAGATC21750.09.4074352
TACCGGT19700.09.15525610
AATCGAA10900.08.8814875
TAGATCC22050.08.8803833
CTAGCCT78450.08.833081
ATCGCTC15500.08.81725442
CTTAGAT25800.08.7823761
GGAAAAC117950.08.6912551
CGGAAAT28150.08.6743941
CCGGAAT34300.08.6583311
ATACGAT35050.08.59541112
ACCGGTG21700.08.51383511
Produced by FastQC (version 0.11.2)