FastQCFastQC Report
Wed 24 May 2017
G152-M6-Index-4_TGACCA-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M6-Index-4_TGACCA-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16488692
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264880.16064342762906844No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT187260.11356874153510782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT23000.016.2589073
TAAGGCG59400.014.4794269
ACCGGTA20200.014.37537527
GTCGGTT33100.014.2075691
ATCCGAA24600.014.12876325
AATCCGA24800.014.103435524
CTCGAAT27750.014.0824571
TCGGTTT33700.013.9686412
AATTGCG21600.013.85093836
ATTGCGA22150.013.20906237
TTGCGAT23000.013.00783738
CACCGGT23350.012.71841426
TGCGATA23350.012.62405139
GTCGGAT9650.012.5835611
AGGCGTC69200.012.4604711
AGGCTAA76950.012.2351525
AAATCCG28250.012.22433223
TTTAGGC72550.012.2190642
AAGGCGT70550.012.19104210
AGTTCCG44700.012.10636226
Produced by FastQC (version 0.11.2)