FastQCFastQC Report
Wed 24 May 2017
G152-M4-Index-9_GATCAG-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M4-Index-9_GATCAG-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15848955
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT162180.10232851314171817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT23850.017.3393923
ACCGGTA20950.015.64576327
AATTGCG21950.014.83247136
ATCCGAA21550.014.291100525
ATTGCGA23150.014.2537137
TAAGGCG54400.014.1929489
CTCGAAT30900.014.1462081
CACCGGT23800.013.9567726
GTCGGTT30050.013.8851531
TGCGATA24050.013.81142839
TTGCGAT24100.013.50928438
AATCCGA22850.012.99664124
CGGGCTA10200.012.9862691
TCGGTTT31700.012.76800252
GTTCGAT27650.012.5353891
AAATCCG24300.012.49203223
CGCGTGT4600.012.4781111
GACACCG27350.012.46688424
TCCGCCC47300.012.32478329
CGCGGGT5600.012.2210071
Produced by FastQC (version 0.11.2)