FastQCFastQC Report
Wed 24 May 2017
G152-M2-Index-2_CGATGT-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M2-Index-2_CGATGT-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222009
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN249970.189055989902896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC10800.014.6682293
CACGATC12150.014.4845461
ACGATCA12150.012.8572872
CCACTAG29350.011.3197743
TTAGATC16900.011.1964152
TAGCTCG27050.010.73563222
TAGCCTC53100.09.9859592
ATCGCTC12000.09.91953442
CGACGAT4358.58563E-109.6084991
TAACCGT8800.09.49432115
CGATGTA3951.7595085E-89.4693634
TAGATCC19200.09.3968343
CTTAGAT19750.09.3562831
AGCTCGT31500.09.21936223
CGTATTA9250.09.03587410
GCTCGTC32900.08.96229524
CTAGCCT62950.08.9111891
CACTAGC37150.08.8838534
TCATACG24300.08.87050110
GATACGC2505.9324515E-48.7999657
Produced by FastQC (version 0.11.2)