FastQCFastQC Report
Wed 24 May 2017
G152-M2-Index-2_CGATGT-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M2-Index-2_CGATGT-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222009
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212100.16041435155580366No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT152470.11531530495857323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT18000.016.7416063
CTCGAAT20900.015.1082991
ACCGGTA15500.014.7603527
AATTGCG15900.014.66562136
CCGCTAA4150.014.31131311
TAAGGCG52350.013.9923739
AATCCGA19600.013.80498124
GTCGGTT25600.013.5420841
TTGCGAT17500.013.45062338
ATCCGAA20550.013.27384325
TGCGATA17700.013.17390339
ATTGCGA17400.013.02220837
CGCTAAA4900.013.01898812
TTACGGC15000.012.75991240
CACCGGT18300.012.74211626
AAATCCG21100.012.71822223
TCGGTTT26700.012.5222212
AGGCGTC57450.012.52049611
CGGGTGT12700.012.3446971
GTCGATT10850.012.210891
Produced by FastQC (version 0.11.2)