FastQCFastQC Report
Wed 24 May 2017
G152-M16-Index-16_CCGTCC-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M16-Index-16_CCGTCC-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15034093
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN271740.18074918121099823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC27750.021.721171
ACGATCA27700.020.8896542
CGATCAC29750.019.5242843
TAATGTA64250.011.3005344
ACGCTAA6250.011.26597726
AATGTAG67700.011.049875
TTAGATC21900.010.64922
TCGCTAG8550.010.55155924
CGCTAGT8600.010.23435425
GGCAACT111300.010.1395311
CGACGAT4855.456968E-129.9787611
CTTTAAT92300.09.8910421
TAATGGG63500.09.79722419
CGAAAAT29250.09.7771681
GCAACTT109200.09.6106112
ACGCAAC8300.09.54109610
TTAATGT82100.09.45996953
ATCGCTC13800.09.4224442
TAGATCC22200.09.4151843
TCACAAG65050.09.4021696
Produced by FastQC (version 0.11.2)