FastQCFastQC Report
Wed 24 May 2017
G152-M15-Index-8_ACTTGA-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M15-Index-8_ACTTGA-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15556801
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290160.18651649526146152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC26100.022.2526261
ACGATCA28000.020.0380212
CGATCAC29550.018.6140023
CGACGAT5500.011.1998751
CGCAATT20550.09.7419841
CGAAAAT31600.09.5378691
TCACAAG62750.09.53636556
GGCAACT111300.09.5272981
CCACTAG27800.09.4971583
TACCGGT19750.09.35516210
ACGCAAC8150.09.17614810
TAGACTT103350.09.0895199
ACTTTAG107750.09.0663565
TAATGTA65500.09.0358334
TTAGACT104000.09.0116438
AATGTAG69900.08.9393975
CTTTAGA109650.08.7880636
GAAAAAC72250.08.7694531
TAGCTCG21500.08.69780322
ACCGGTG21850.08.65709311
Produced by FastQC (version 0.11.2)