FastQCFastQC Report
Wed 24 May 2017
G152-M15-Index-8_ACTTGA-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M15-Index-8_ACTTGA-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15556801
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT183760.11812197122017566No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT176470.11343591783426424No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC175870.11305023442801641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA21800.015.13634927
CGGGATA9800.015.0969161
CGAATGT23100.015.0456353
CTCGAAT26900.014.2835611
ATTGCGA23450.013.97768937
ATCCGAA19400.013.947062525
AATTGCG23250.013.81398736
TGCGATA23850.013.4661239
AATCCGA20900.013.36708824
CGCGTGT4800.013.3412431
TTGCGAT24900.013.16372638
CACCGGT25100.013.14609926
CGGGGAT16900.012.804981
GTCGGAT7700.012.6183191
GTCGCAA3501.8189894E-1212.6183181
TCGGTTT31050.012.6102532
GTCGGTT29250.012.3810511
GACACCG26950.012.24367724
TACGTCA71000.012.17730832
ACGTCAC72850.011.92861933
Produced by FastQC (version 0.11.2)