FastQCFastQC Report
Wed 24 May 2017
G152-M14-Index-7_CAGATC-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M14-Index-7_CAGATC-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15617230
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274230.17559451964272796No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT181840.11643550104596015No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT172890.11070465120895318No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC171770.1099874945813054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA19200.019.47785427
AATTGCG20700.017.85375436
ATTGCGA21050.017.66157237
TTGCGAT23350.016.11031338
CACCGGT23850.015.95673926
TCGGTTT30850.015.61552
TGCGATA24000.015.58168439
CGGGATA8750.015.3921471
GTCGGTT30950.015.1948471
CCGGTAG25250.015.07225128
GACACCG26850.014.58351224
AATCCGA19650.014.55349324
CGAATGT21850.014.4969963
ATCCGAA20500.014.16467225
TAAGGCG40350.014.11949
CGGTAGG27300.013.7792929
CGGTTTG32900.013.6395863
GCGATAA28250.013.31621540
AAATCCG21200.013.28080523
ACACCGG29000.013.27475525
Produced by FastQC (version 0.11.2)