FastQCFastQC Report
Wed 24 May 2017
G152-M13-Index-15_ATGTCA-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M13-Index-15_ATGTCA-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21706646
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN422110.19446118023023914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC37500.023.699811
ACGATCA41550.020.7048992
CGATCAC46150.018.5933763
TTAGATC28750.011.2498532
GGCAACT161700.010.6387721
TAATGTA88700.010.24454
GCAACTT157650.010.1183892
TCACAAG90000.010.0955876
TAGATCC31050.09.991323
ATCGCTC20150.09.95526942
TAGACTT156550.09.9494059
CGACAAT13500.09.9401031
AATGTAG94900.09.8766755
ACTTTAG162950.09.8702995
TTAGACT157500.09.847658
CGCAATT26100.09.777151
CTTTAGA164300.09.5739786
GTCTTGG166750.09.44212234
ATCCATG163400.09.39959228
TTTAGAC165550.09.355517
Produced by FastQC (version 0.11.2)