FastQCFastQC Report
Wed 24 May 2017
G152-M12-Index-14_AGTTCC-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M12-Index-14_AGTTCC-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16128600
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN313530.19439380975410142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC26400.020.9146271
ACGATCA27000.019.8823382
CGATCAC28250.018.9243533
CGTAGCG2452.240995E-812.5632620
CGACGAT4950.010.6656271
TTAGATC21800.09.688492
TAGCCTC55400.09.5708082
CGACAAT10600.09.3387121
GGCAACT106950.09.3174641
ACGACGC16350.09.27987737
CGCAATT20950.09.1351471
CGTACCG3157.5937023E-69.07496636
TCATACG27400.09.0712310
ACCCGCG4153.93793E-89.0118166
TCACAAG64900.08.98282056
TTAGACT94550.08.9114588
CTAGCCT63400.08.8477171
CACGACG17200.08.82130536
CCACTAG27700.08.8160983
TTAACGA47150.08.7707121
Produced by FastQC (version 0.11.2)