FastQCFastQC Report
Wed 24 May 2017
G152-M10-Index-6_GCCAAT-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M10-Index-6_GCCAAT-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20756347
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN373820.18009912823292076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC32150.019.7058281
CGATCAC31450.018.887743
ACGATCA33250.018.3285962
CGACAAT14700.010.77452851
CCACTAG40600.010.2417143
GGCAACT114050.09.9526221
TTAGATC26450.09.5656222
ACGCAAC11850.09.46639110
ACTTTAG108800.09.4232395
TAATGTA67050.09.4171374
TTAGACT101750.09.4050738
CTTAGAT32150.09.305531
GCAACTT111150.09.2833442
CGCAATT25200.09.2531251
TAGCTCG33100.09.238255522
AATGTAG73650.09.1708345
TAGCCTC77700.09.1458192
TAGACTT105550.09.1288679
CTTTAGA109800.09.0761826
CACGATA32850.09.05894842
Produced by FastQC (version 0.11.2)