FastQCFastQC Report
Wed 24 May 2017
G152-M10-Index-6_GCCAAT-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M10-Index-6_GCCAAT-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20756347
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263520.12695875627826034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA27050.017.32210427
CGAATGT26550.016.321683
AATTGCG30200.015.224400536
TAAGGCG54050.015.0988369
ATCCGAA30650.014.64122925
ATTGCGA31550.014.5729637
TCGGTTT38800.014.4000882
CACCGGT33800.014.25296526
CGGGATA12950.014.1500291
AATCCGA31750.014.13397224
GTCGGTT39100.014.1160211
CTCGAAT35750.014.0801681
TTGCGAT33250.013.960238
AAATCCG32300.013.89239923
CCGGTAG34300.013.7248628
TGCGATA34350.013.57660439
GACACCG36350.013.37413324
TGCTGCG38000.012.56149711
AGGCGTC64750.012.50182211
AAGGCGT68350.012.29391310
Produced by FastQC (version 0.11.2)