FastQCFastQC Report
Fri 5 May 2017
G150-M19-Index-20_GTGGCC-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M19-Index-20_GTGGCC-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21657223
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13650.023.82208644
TCTCGTA13700.022.93163943
GATCGGA15450.021.6795541
ATCGGAA18200.018.8751262
TATCTCG16300.018.7337141
CGTCTGA20700.016.59467716
ATCTCGT18950.016.461742
ACACGTC21200.016.41079913
ACGTCTG24700.014.35209715
TCGGAAG26550.013.27049353
GTCACGT25500.013.01153829
AGTCACG26650.012.86130828
ACGTGGC26900.012.41684532
CGGAAGA29500.011.8693734
CACGTCT30100.011.85072614
CGTGGCC30000.011.4267933
CACACGT39550.09.40907112
TCACGTG37750.09.25503430
GCACACG44200.08.41863711
CACGTGG45650.07.846333531
Produced by FastQC (version 0.11.2)