FastQCFastQC Report
Fri 5 May 2017
G150-M18_thru_21.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M18_thru_21.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92642321
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA52950.020.435461
CTCGTAT51800.020.27455144
TCTCGTA53400.018.80152543
ATCGGAA61000.017.8337942
CCGTACG13750.016.30107333
CGTACGT15550.014.97944934
CGTCTGA75000.014.76757416
ACACGTC79650.014.26462913
ATCTCGT72150.014.0364442
CGTTTCG14500.013.48778733
ACGTCTG93000.012.2872715
TCGGAAG96550.011.5177313
AGTCACG84950.010.55230928
CGGAAGA108250.010.3955194
GTCACGT86500.010.38959829
CACGTCT117450.09.91704514
ACCGTAC26000.09.04331732
AATCTCG45100.08.96264641
AACGATC31850.08.62191938
CGTAATC25200.08.45618738
Produced by FastQC (version 0.11.2)