FastQCFastQC Report
Fri 5 May 2017
G150-M16-Index-16_CCGTCC-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M16-Index-16_CCGTCC-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23557228
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT6350.025.98950411
CGTCGTG6400.025.44264212
AGCGTCG7200.022.6156810
GATCGGA11150.015.5882121
GTCGCCG9100.014.50710244
ATCGGAA13250.012.9516092
TCGTGTA13800.012.915297514
GAGCGTC14000.012.1022669
GTCGTGT14850.011.70599513
CGTGTAG16350.010.90096215
TGGTCGC12450.010.60194142
GATCTCG13650.010.31366434
GGTCGCC13800.09.56599343
GTGGTCG15300.09.05765441
AGAGCGT19950.09.0441668
AAGAGCG22400.08.6444217
TCGGAAG22000.07.90099333
ATCTCGG18800.07.839263435
CGGAAGA25550.07.23380044
TCGGTGG22850.07.123564738
Produced by FastQC (version 0.11.2)