FastQCFastQC Report
Fri 5 May 2017
G150-M15_thru_21.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M15_thru_21.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162404554
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA93200.019.0579971
CTCGTAT87350.018.33598544
TCTCGTA86550.017.51401943
ATCGGAA108000.016.7810172
CGTCTGA131950.014.01795416
ACACGTC137150.013.27762913
ATCTCGT125450.012.53775942
CCGTACG17600.012.11059833
ACGTCTG155400.011.78846215
TCGGAAG164250.011.3421933
ACGTCCG27600.010.82767632
CGTTTCG21800.010.78533833
CGGAAGA188900.09.9676764
CGTACGT22600.09.52854334
CACGTCT206150.09.12149614
CGTCCGC33500.09.05192133
AATCTCG85250.08.65815641
GTCACGT140850.08.439527529
AGTCACG143350.08.21492628
CACACGT264000.07.39825612
Produced by FastQC (version 0.11.2)