FastQCFastQC Report
Fri 5 May 2017
G150-M4-Index-4_TGACCA-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M4-Index-4_TGACCA-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18344229
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14300.025.37852543
CTCGTAT14350.024.67828442
GATCGGA15350.024.5203111
CGTATGC15050.024.26015744
TCTCGTA14800.023.92825541
ATCGGAA16750.021.7988822
AATCTCG16050.021.51696239
ATCTCGT17750.019.20770640
ACACGTC19600.018.96871613
CGTCTGA20350.018.26713216
ACGTCTG25050.015.10311115
TCGGAAG25450.014.6890733
CGGAAGA28600.013.4560354
CACGTCT30000.012.68542114
CACACGT32250.012.07399112
GCACACG37550.010.31121911
GACCAAT38100.09.75916935
ACCAATC37400.09.47100236
CCAATCT47850.07.90808937
CAATCTC49400.07.48074138
Produced by FastQC (version 0.11.2)