FastQCFastQC Report
Fri 5 May 2017
G150-M1_thru_7.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M1_thru_7.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139099852
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT109100.026.55264742
TCGTATG111650.026.31890343
TCTCGTA111750.024.91891741
GATCGGA126400.024.1519131
CGTATGC123900.023.87682244
ATCGGAA132300.023.2421022
ATCTCGT135900.020.47424340
ACACGTC161750.018.83709713
CGTCTGA163900.018.6010516
ACGTCTG187950.016.37296315
TCGGAAG209500.014.8315973
CACGTCT221100.014.11801614
CGGAAGA233850.013.3535354
CACACGT275350.011.6487712
TATCTCG73950.011.57085639
CGCCAAT58450.010.68991733
GCACACG309550.010.58917311
CATCTCG127850.08.03471139
GATCTCG94900.07.741665439
ACGCCAA90450.07.321347732
Produced by FastQC (version 0.11.2)