FastQCFastQC Report
Wed 31 Aug 2016
BM-S1-15_S39_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBM-S1-15_S39_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21797312
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10150.022.58945542
TCTCGTA10850.020.71795341
TCGTATG10800.020.60577843
CGTATGC13200.017.0295744
GATCGGA13900.016.9424741
ATCGGAA15300.015.1334972
AATCTCG14000.015.09240639
CGTCTGA17950.013.02161216
ACACGTC18050.012.82495613
ATCTCGT18450.011.69592440
ACGTCTG21000.011.66549215
TCGGAAG25300.09.417253
CGGAAGA27950.08.6047984
CACGTCT28650.08.31528514
GTATGCC30800.08.10057745
CACACGT35900.06.82382612
GCACACG37900.06.404429411
GTAATCT38750.05.975455337
Produced by FastQC (version 0.11.2)