FastQCFastQC Report
Wed 31 Aug 2016
BM-S1-12_S36_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBM-S1-12_S36_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19180279
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA8250.020.16293141
CTCGTAT9050.019.37391542
TCGTATG9500.018.45634843
GATCGGA9850.017.9375551
ACGATCA11100.015.7982832
ATCGGAA11700.015.3701772
CGATCAG11200.015.25575633
GTCACGA12450.014.8048329
CGTATGC12500.014.56631844
TCACGAT13900.013.42250330
CGTCTGA13900.013.42005416
CACGATC12900.013.2451731
ACACGTC15950.011.83612613
GATCTCG15200.011.23876939
AGTCACG18500.010.8134928
ACGTCTG18400.010.74869715
ATCTCGT16350.010.44830240
TCGGAAG20350.09.2776893
CGGAAGA21850.08.846514
CACGTCT25650.08.06104714
Produced by FastQC (version 0.11.2)