FastQCFastQC Report
Wed 31 Aug 2016
BM-S1-10_S34_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBM-S1-10_S34_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33722374
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA12750.019.0137711
CTCGTAT12600.018.19709244
TCGTATG13550.017.41803745
TCTCGTA13050.017.39735443
GTCACGA14550.016.68514329
ACGAGTG18450.014.0132332
ATCGGAA17600.013.9221042
CGTCTGA18800.013.38955716
CGAGTGG19750.013.09083933
CACGAGT19350.012.8965931
ATCTCGT18300.012.7745542
TCACGAG19700.012.0953530
ACGTCTG21800.011.65005515
TATCTCG21000.011.34632241
AGTCACG23800.010.76662628
TCGGAAG23450.010.2562553
ACACGTC26250.09.33261313
CGGAAGA26650.09.1934224
GCACACG39350.06.4541611
GGATATC42050.06.30767138
Produced by FastQC (version 0.11.2)