FastQCFastQC Report
Fri 24 Mar 2017
G144_M7M8.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144_M7M8.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66536526
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT31450.011.810225544
TCTCGTA35850.09.68647243
ATCTCGT48300.07.18973542
ATCGACG9651.1459633E-106.37744541
AACGATC33100.06.04278638
ACGACGC30250.05.90001916
AGTCACG75100.05.56162628
TTACGCG12402.764864E-105.508505316
CACGACG32100.05.49146915
TACGCGA11752.3173925E-95.437958717
TACACGA37150.05.33832413
ACGATCT40750.05.12412239
GTCACGT81150.05.119737629
TGAAACG50850.05.01451235
Produced by FastQC (version 0.11.2)