FastQCFastQC Report
Fri 24 Mar 2017
G144_M7-M10.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144_M7-M10.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146036138
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT66950.010.30774644
TCTCGTA75300.08.46432743
ACGACGC68400.07.087121516
CACGACG74600.06.380097415
ATCTCGT103250.06.1731142
TACACGA84250.06.06809713
TCCGATC82250.06.06742126
CCGTACG34400.05.7510333
TACGCGA26350.05.68612317
CTACACG95350.05.6621412
TTACGCG26750.05.601294516
ATCGACG22600.05.543422741
CGGTTTT167950.05.53462651
CGACGCT81950.05.484905717
CCGATCT92250.05.242917527
TTCCGAT103100.05.159928325
CGTACGT39700.05.09399934
Produced by FastQC (version 0.11.2)