FastQCFastQC Report
Wed 7 Dec 2016
G144-M9-Index-21_GTTTCG_BC9WPLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144-M9-Index-21_GTTTCG_BC9WPLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35978029
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT10206.730261E-116.2557331
ATCGTCG25450.05.449228324
ATCGACG7701.8408076E-55.430787641
ACGATCG7008.144585E-45.02818733
Produced by FastQC (version 0.11.2)