FastQCFastQC Report
Thu 12 Jan 2017
G143_M7-M12.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143_M7-M12.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157352836
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG300450.036.05038543
CGTATGC308350.035.1622444
CGTCTGA572950.028.64595416
ACGTCTG604450.027.2152415
CGATCAG163500.027.2036433
ACACGTC612050.026.84958813
ACGATCA171800.026.13310632
CCGTATC113650.025.62410538
CTCGTAT414500.025.39993142
TCTCGTA405100.025.03529241
GTCACGA179750.025.0140629
AGTCACG299950.024.6139628
ATCGGAA669100.024.4041752
CACGTCT691000.023.94310214
GATCGGA686000.023.8061641
TCACGAT192450.023.38617930
TCGGAAG704400.023.2970163
TCCGTAT127800.023.2685537
CGGAAGA705400.023.217354
CGTATCT126550.023.13352439
Produced by FastQC (version 0.11.2)