FastQCFastQC Report
Thu 12 Jan 2017
G143-M9-Index-11_GGCTAC_BC9WPLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M9-Index-11_GGCTAC_BC9WPLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21603624
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC586790.27161646582999227TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT70350.039.21259342
TCGTATG72200.039.0596143
CGTATGC72350.038.9783644
TCTCGTA69200.038.4680941
CGGCTAC76450.037.1803233
ACGGCTA76700.036.9458632
ATCTCGT75550.035.2933940
TCACGGC83450.034.11553230
CATCTCG78100.034.0573839
AGTCACG86350.033.0226528
GTCACGG87400.032.49829529
CACGGCT88100.032.33966431
CGTCTGA87800.032.30090316
ACGTCTG91100.031.37309815
ACACGTC93450.030.53763213
CACGTCT104800.027.37644214
ATCGGAA109000.026.211512
CACACGT109700.026.15440212
GATCGGA110200.025.9527021
GCACACG111800.025.74155211
Produced by FastQC (version 0.11.2)