FastQCFastQC Report
Wed 7 Dec 2016
G143-M7-M14-Index-8_ACTTGA_BC9WPLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M7-M14-Index-8_ACTTGA_BC9WPLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42160107
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG2141570.5079612345386125Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT1477490.3504474028018952Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGAACTCTGAAC901560.2138419620234835Illumina DpnII expression Sequencing Primer (100% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG895650.21244016292463397Illumina Single End PCR Primer 1 (100% over 50bp)
TGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC821460.19484295900861923Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA712200.1689274650085684Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA707190.16773913785370612No Hit
GATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT605510.14362155200412563Illumina RNA PCR Primer (100% over 31bp)
GGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAAC564300.1338469088799988No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAGAACTCTGAACGT541930.12854094511667155Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGGGCTCGTCCCTGTTTCCGGAGATCGTCGGACTGTAGAACTCTGAACG499360.11844372216607514Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT471200.11176442222976331Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGT456460.10826822616935008Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC450360.10682136077121436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG88650.038.5593313
CGACCGT89250.038.37219214
CGTCGTG128100.036.5236312
GCGTCGT128100.036.42920311
GTAGTGC237750.035.89243711
GTTCGAC96600.035.48066311
GTCGTGT135000.034.94408813
TTCGACC98750.034.84993712
TCGTGTA133450.034.5633714
AGCGTCG133750.034.48702210
AAGTTCG100050.034.3182149
GACCGTC103450.033.4512115
TAGTGCA256650.033.2914212
ACCGTCT107550.032.10084516
TGTAGTG271050.031.65983810
GAGCGTC145000.031.6536129
AGAGCGT145600.031.6384328
AGTGCAC272900.031.34263213
GTGCACT277200.030.9938314
CCGTCTT111600.030.77991717
Produced by FastQC (version 0.11.2)