FastQCFastQC Report
Thu 12 Jan 2017
G143-M12-Index-14_AGTTCC_BC9WPLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M12-Index-14_AGTTCC_BC9WPLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27190628
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT638410.23479045794749573TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT76450.039.585344
TCTCGTA76400.038.14533243
CCGTATC76200.038.1315738
TCCGTAT79850.037.46184537
TATCTCG78600.037.02190841
CGTATCT78650.036.9715339
ATCTCGT82700.035.26601442
AGTTCCG90000.034.7512534
TTCCGTA91900.033.86492536
GTTCCGT95150.032.7317235
CGTCTGA103200.030.8644716
ACGTCTG110350.029.04488415
ACACGTC112650.028.4336413
CACGTCT126200.025.37853414
GTATCTC115850.025.3647840
CACACGT133150.024.18804212
ATCGGAA134000.023.555972
GCACACG138350.023.08710911
TCGGAAG137900.022.9696853
CGGAAGA138600.022.8061244
Produced by FastQC (version 0.11.2)