FastQCFastQC Report
Thu 12 Jan 2017
G143-M11-Index-13_AGTCAA_BC9WPLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M11-Index-13_AGTCAA_BC9WPLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21930173
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT437740.1996062684959211TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT57800.038.37842644
TCTCGTA55350.037.81550643
AATCTCG58950.035.58067341
ATCTCGT63850.032.91892642
CGTCTGA73750.032.80011716
ACGTCTG76800.031.41129315
ACACGTC79450.030.5037513
ATCGGAA89000.026.9547062
GATCGGA89550.026.6989381
CACGTCT91800.026.4462414
CACACGT95250.025.6981212
CGGAAGA94150.025.3872174
TCGGAAG94950.025.265663
AGTCAAC95250.025.02032934
GCACACG98000.024.841311
GTCAACA98650.024.2247435
AACAATC95950.022.34160238
CAGTCAA110150.021.95588333
ACAATCT98850.021.59708439
CAATCTC98950.021.50837140
Produced by FastQC (version 0.11.2)