FastQCFastQC Report
Sun 7 Aug 2016
GBM85.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM85.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85402425
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC331750.026.7082129
CATACGA132700.025.53296714-15
AAGACGG363350.024.4718828
GCATACG158950.024.3937414-15
CGGCATA165600.023.10178212-13
ATACGAG151850.020.06777816-17
GAAGACG459400.019.1211577
ACGGCAT271200.014.02048910-11
CAAGCAG969500.013.8445441
GACGGCA326000.012.02149310-11
GGCATAC348750.011.43928712-13
TACGAGA210450.011.43036816-17
CCCGTAT120600.010.8989651
GATCGGA96150.010.6377671
CGTATCG90450.010.4644863
CGCGTAA41750.010.3152481
AAGCAGA1266450.010.1797792
AGAAGAC941000.010.1116166
GTATCGA90550.09.8088694
ATCGAAG227650.09.675726
Produced by FastQC (version 0.11.2)