FastQCFastQC Report
Sun 7 Aug 2016
GBM85.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM85.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85402425
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG88850.011.4973783
CCCGTAT117250.010.6718121
ATCGAAG230550.09.6851896
GTATCGA90250.09.6161364
CCGTATC135850.08.9836312
CCGGTAT162600.08.4481961
CTCGGAT213650.08.3181181
TGCGCGA85100.08.2859419
CCCGGAT252650.08.2543041
CGGTATT154150.08.1223952
CGCGAAT30200.08.1064131
CTCGCAT223000.07.88803431
CTCGGGT256950.07.8691561
CTCCGAT137150.07.7019741
CTCGGTT135250.07.6090511
TATCGAA122650.07.5918215
CGGGAAT177250.07.5708981
CTGGAAT886600.07.52700141
TCGAAGG291950.07.52443847
CTCGACT148400.07.5152451
Produced by FastQC (version 0.11.2)