FastQCFastQC Report
Sun 7 Aug 2016
GBM84.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM84.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254234
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA82270.364957675201421No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA49680.22038528387026368No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG42610.18902208022769595No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC31890.14146712364377434No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTC31840.14124531880896127No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA26320.11675806504559864No Hit
GTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGA25720.11409640702784182No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA24710.10961594936461785No Hit
CACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTT23560.10451443816391733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC3200.054.61719
GATCGGA3500.052.4858861
CAGAAGA4650.052.0328035
AGAGCGT2450.051.216038
CAAGCAG3500.051.205741
TCGGAAG3300.050.2348183
CCTGCCA3850.047.7144471
TCAGACG8750.045.5720942
ACGTATT207.149319E-444.85360320-21
TGCCGTA6600.044.8040242
CTGCCGT7000.044.164951
GTTAGGA2350.043.8517231
CGGAAAG2750.040.7327465
TGTTACG3300.040.730933
CAGTTTT10850.040.468153
TTCGGTC803.5806625E-739.2035182
CTTGTTA3700.038.7502941
CTGCCAG6650.038.404311
GTATCAT1750.037.79904650-51
CCTCACC13400.037.783341
Produced by FastQC (version 0.11.2)