FastQCFastQC Report
Sun 7 Aug 2016
GBM84.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM84.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254234
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA41830.18556192480461212No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCNA40270.1786416139584444No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG25070.11121294417527196No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATNG24840.11019264193513184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCC158.224343E-489.947363
GCGTATC158.224343E-489.947364
TACGTTC256.917467E-571.957893
CCGCGAT200.002545164767.667821
CTTATAG301.6797586E-460.149181
GTTAGGA1350.060.1491781
TTACGTT301.7054382E-459.964912
CGTGGTA651.0895747E-955.352223
CCTGCCA2700.055.1367451
GCTCGAC250.006159023454.1342621
CTTACGT250.006159023454.1342621
GGCGTAT250.00623423453.968423
TAGTACG250.00623423453.968427
TGCCGTA3700.053.4822122
CGTGGTT353.597303E-451.556441
TCAGACG9450.051.398492
CGCGTTC353.6521943E-451.398493
GTAGTAT353.6521943E-451.398498
CACGACT452.0576716E-550.124311
CTGCCGT4250.048.826981
Produced by FastQC (version 0.11.2)