FastQCFastQC Report
Sun 7 Aug 2016
GBM61.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM61.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326109
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA3350.044.022681
TCGTGCG2400.042.814617
TGCCGTA2350.041.8165052
GGCTCGT2500.041.1004644
TCCCGTC2050.039.2203642
CTGCCGT2750.037.376831
CTCGAAC601.1873992E-437.241411
TGTTACG3650.036.7200933
GTCCCGT2700.033.092166
GTTACGA4100.032.68864
TTACGAC4250.031.5348855
CCTCACC14650.031.4200531
GTGCGTC3150.031.2094759
TACGACT4450.030.1175866
GTTAGGA1352.1682354E-829.7931251
TCCCGTT3150.029.7840757
CTGCCAG6200.029.5528561
AGGCGTA2900.029.2772278
ACCCGAA1253.6846177E-728.5927147
GCTCGTG3450.028.4880355
Produced by FastQC (version 0.11.2)