FastQCFastQC Report
Sun 7 Aug 2016
GBM6.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM6.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83439125
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG88300.012.28681853
CCCGTAT117950.012.1468951
TGCGCGA79550.011.7636739
CTCGGAT222300.011.3051861
CTCCGAT187300.010.9599121
CTCGTAT99750.010.7370591
CTCGGTT153300.010.2345271
CTCAGAT612900.010.2012141
TCGATCG72950.010.1205868
GTATCGA100750.09.9284344
CTTGGAT644750.09.602571
CTCGAAT202900.09.5153151
CTCGCAT229500.09.4972681
ACGTGCG131150.09.4618256
CAATCGG113800.09.4501664
CGATCGG78350.09.4230339
ATCGAAG250950.09.42156
CTGGAAT911850.09.324351
GACGCTA88200.09.104874
TTCGCGC61750.09.0623547
Produced by FastQC (version 0.11.2)