FastQCFastQC Report
Sun 7 Aug 2016
GBM5.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM5.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80447447
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG69300.011.6056293
TACCGTA70950.011.6006349
CCCGTAT124400.010.1734151
CTCGGAT212500.09.7858421
CTTGGAT567050.09.31736851
CTACCGT101400.08.9982878
CTCGTAT110500.08.8133891
GACGCTA78600.08.7961154
CCGGGAT210200.08.7066631
CCGTATC118200.08.5955172
GTATCGA97300.08.5557194
CAATCGG94350.08.3745894
CTGGAAT862850.08.3042321
CTCGGTT133650.07.9908281
CGGGTAT67000.07.8646271
CGAAGAT254050.07.83350321
CTCAGAT600850.07.7361651
TCAATCG92650.07.6654443
CTCGGGT220250.07.64719151
ACTACCG117700.07.6322587
Produced by FastQC (version 0.11.2)