FastQCFastQC Report
Sun 7 Aug 2016
GBM44.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM44.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699621
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG1650.039.6946753
GTTACGA1800.038.9858444
TTACGAC1850.037.932175
GCCGTAT1251.5825208E-1037.4264073
TGCCGTA1256.548362E-933.6837652
CCGAAAG1151.1780685E-732.5611881
CCGCTGT600.004244589731.2044731
CCACGAG1201.7114326E-731.2044731
ACGAGCG1201.7190541E-731.1886773
CGGTAAA2150.030.4655599
CGGAAAC1551.5861588E-930.1978781
CGTATCG1551.5934347E-930.182595
ATCCGGT2200.029.771016
TCCGCTT953.8704344E-529.5471672
ACTATGC2550.029.3540485
TCGTGCG804.910169E-429.2393847
AACGCAT650.006293153428.7895475
TTATCCG2450.028.6426624
CCGGTAA2300.028.4786768
AGGCGTA1653.106834E-928.3553928
Produced by FastQC (version 0.11.2)