FastQCFastQC Report
Sun 7 Aug 2016
GBM43.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM43.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53527052
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG57600.015.1522323
CCCGTAT90150.013.6903421
GTATCGA73750.013.2336534
CCGTATC99100.010.9376512
CTCGGAT141300.010.3286261
CTCCGAT116300.09.3612221
CTTGGAT391300.09.0904141
CGCGTAT13700.08.9058851
ATCGAAG146150.08.9015276
TATCGAA104050.08.8378735
CTCGGTT97200.08.7384941
CTCAGAT380950.08.7091481
CTCGAAT126850.08.5826591
TCGGATT85250.08.4213712
CTGGAAT575450.08.3587351
CTCGCAT123000.08.2790191
TACCGTA48200.08.0874549
TAAGGCG90350.08.0572628
CTCGTTT112800.08.0292041
CTCGTAT58450.07.8680271
Produced by FastQC (version 0.11.2)